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Cellosaurus 6647 (CVCL_H722)

Cell line name 6647
Synonyms #6647
Accession CVCL_H722
Resource Identification Initiative To cite this cell line use: 6647 (RRID:CVCL_H722)
Comments Omics: Array-based CGH.
Omics: Transcriptome analysis by RNAseq.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene fusion; HGNC; 3508; EWSR1 + HGNC; 3749; FLI1; Name(s)=EWSR1-FLI1, EWS-FLI1; Note=Type 2 fusion (Ex7/Ex5) (PubMed=8040301; PubMed=11891184; PubMed=25010205).
  • Mutation; HGNC; 11998; TP53; Simple; p.Ser241Phe (c.722C>T); ClinVar=VCV000012359; Zygosity=Unspecified (PubMed=25010205).
Disease Ewing sarcoma (NCIt: C4817)
Ewing sarcoma (ORDO: Orphanet_319)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 14Y
Category Cancer cell line
STR profile Source(s): PubMed=25010205

Markers:
AmelogeninX
CSF1PO11,13
D2S133817,25
D3S135815,17
D5S81811,13
D7S8208,10
D8S117910,13
D13S3178
D16S53910,12
D18S5113,19
D19S43314,14.2
D21S1129,30
FGA22,24
TH019,9.3
TPOX8,9
vWA14,18

Run an STR similarity search on this cell line
Publications

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=3004699; DOI=10.1016/0165-4608(86)90001-4
Whang-Peng J., Triche T.J., Knutsen T., Miser J.S., Kao-Shan S., Tsai S., Israel M.A.
Cytogenetic characterization of selected small round cell tumors of childhood.
Cancer Genet. Cytogenet. 21:185-208(1986)

PubMed=3390826
McKeon C., Thiele C.J., Ross R.A., Kwan M., Triche T.J., Miser J.S., Israel M.A.
Indistinguishable patterns of protooncogene expression in two distinct but closely related tumors: Ewing's sarcoma and neuroepithelioma.
Cancer Res. 48:4307-4311(1988)

PubMed=8040301; DOI=10.1172/JCI117360
Giovannini M., Biegel J.A., Serra M., Wang J.-Y., Wei Y.-L.H., Nycum L., Emanuel B.S., Evans G.A.
EWS-erg and EWS-Fli1 fusion transcripts in Ewing's sarcoma and primitive neuroectodermal tumors with variant translocations.
J. Clin. Invest. 94:489-496(1994)

PubMed=8617485; DOI=10.1016/S0046-8177(96)90115-X
Scotlandi K., Serra M., Manara M.C., Benini S., Sarti M., Maurici D., Lollini P.-L., Picci P., Bertoni F., Baldini N.
Immunostaining of the p30/32MIC2 antigen and molecular detection of EWS rearrangements for the diagnosis of Ewing's sarcoma and peripheral neuroectodermal tumor.
Hum. Pathol. 27:408-416(1996)

PubMed=11891184; DOI=10.1016/S0002-9440(10)64908-5
Manara M.C., Perbal B., Benini S., Strammiello R., Cerisano V., Perdichizzi S., Serra M., Astolfi A., Bertoni F., Alami J., Yeger H., Picci P., Scotlandi K.
The expression of ccn3(nov) gene in musculoskeletal tumors.
Am. J. Pathol. 160:849-859(2002)

PubMed=18160777; DOI=10.1159/000109614
Savola S., Nardi F., Scotlandi K., Picci P., Knuutila S.
Microdeletions in 9p21.3 induce false negative results in CDKN2A FISH analysis of Ewing sarcoma.
Cytogenet. Genome Res. 119:21-26(2007)

PubMed=22142829; DOI=10.1158/1078-0432.CCR-11-2056
Shukla N., Ameur N., Yilmaz I., Nafa K., Lau C.-Y., Marchetti A., Borsu L., Barr F.G., Ladanyi M.
Oncogene mutation profiling of pediatric solid tumors reveals significant subsets of embryonal rhabdomyosarcoma and neuroblastoma with mutated genes in growth signaling pathways.
Clin. Cancer Res. 18:748-757(2012)

PubMed=25010205; DOI=10.1371/journal.pgen.1004475
Brohl A.S., Solomon D.A., Chang W., Wang J.-J., Song Y., Sindiri S., Patidar R., Hurd L., Chen L., Shern J.F., Liao H.-L., Wen X.-Y., Gerard J., Kim J.-S., Lopez Guerrero J.A., Machado I., Wai D.H., Picci P., Triche T.J., Horvai A.E., Miettinen M.M., Wei J.S., Catchpoole D., Llombart-Bosch A., Waldman T., Khan J.
The genomic landscape of the Ewing sarcoma family of tumors reveals recurrent STAG2 mutation.
PLoS Genet. 10:E1004475-E1004475(2014)

PubMed=26428435; DOI=10.1016/j.ejca.2015.08.020
Sand L.G.L., Scotlandi K., Berghuis D., Snaar-Jagalska B.E., Picci P., Schmidt T., Szuhai K., Hogendoorn P.C.W.
CXCL14, CXCR7 expression and CXCR4 splice variant ratio associate with survival and metastases in Ewing sarcoma patients.
Eur. J. Cancer 51:2624-2633(2015)

Cross-references
Cell line databases/resources cancercelllines; CVCL_H722
Encyclopedic resources Wikidata; Q53996682
Polymorphism and mutation databases Cosmic; 1078384
Cosmic; 1082508
Cosmic; 1718435
Cosmic; 2228212
Entry history
Entry creation11-Feb-2013
Last entry update05-Oct-2023
Version number25