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Cellosaurus IMR-90/GFPdeltaRaf-1[YY]:ER (CVCL_HA48)

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Cell line name IMR-90/GFPdeltaRaf-1[YY]:ER
Accession CVCL_HA48
Resource Identification Initiative To cite this cell line use: IMR-90/GFPdeltaRaf-1[YY]:ER (RRID:CVCL_HA48)
Comments Characteristics: Transfected with a construct that is composed of EGFP at the N-terminus, the catalytic domain of RAF1 (with 340-DD-341) in the middle and the hormone-binding domain of ESR1 at the C-terminus. Protein kinase activity is activated by estrogen or analogs such as 4-hydroxy-tamoxifen.
Transfected with: HGNC; 9829; RAF1 (catalytic domain with D-340 and D-341).
Transfected with: HGNC; 3467; ESR1 (hormone-binding domain).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Derived from sampling site: Fetal lung.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0347 (IMR-90)
Sex of cell Female
Age at sampling 16FW
Category Finite cell line

PubMed=9765202; DOI=10.1101/gad.12.19.2997
Zhu J., Woods D., McMahon M., Bishop J.M.
Senescence of human fibroblasts induced by oncogenic Raf.
Genes Dev. 12:2997-3007(1998)

Other Wikidata; Q54897673
Entry history
Entry creation26-Sep-2016
Last entry update12-Mar-2020
Version number7