| Cell line name | NIH 3T3 Duo-BFP53BP1 |
|---|---|
| Accession | CVCL_HC52 |
| Resource Identification Initiative | To cite this cell line use: NIH 3T3 Duo-BFP53BP1 (RRID:CVCL_HC52) |
| Comments | Characteristics: Cell developed for the induction and visualization of double-strand breaks chromosomal damage at specific sites of the mammalian genome. Sytem based on the stable integration of endonuclease ISceI recognition sites flanked by LacO/TetO operator arrays, coupled with retroviral-mediated integration of their fluorescent repressors (LacR/TetR) to visualize the LacO/TetO sites. Characteristics: Integrates one copy of a LacO-ISceI construct (256 copies of LacO), two copies of a TetO-ISceI-TetO construct (96 copies of TetO) as well as a LacR-GFP and a TetR-mCherry constructs. Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein. Transfected with: UniProtKB; Q8ISF8; Entacmaea quadricolor eqFP611 (modified to produce the BFP mTagBFP). Transfected with: UniProtKB; P03023; Escherichia coli lacI. Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. Transfected with: MGI; MGI:1351320; Trp53bp1. Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922. Cell type: Fibroblast; CL=CL_0000057. |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss. |
| Hierarchy | Parent: CVCL_HC51 (NIH 3T3 Duo) |
| Sex of cell | Male |
| Age at sampling | Embryo |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=23929981; DOI=10.1126/science.1237150 |
| Cross-references | |
| Cell line collections (Providers) | Kerafast; ENH104-FP |
| Encyclopedic resources | Wikidata; Q54930577 |
| Entry history | |
| Entry creation | 01-Dec-2016 |
| Last entry update | 29-Jun-2023 |
| Version number | 12 |