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Cellosaurus HCT 116 CDKN1A(-/-) (CVCL_HD73)

[Text version]
Cell line name HCT 116 CDKN1A(-/-)
Synonyms CDKN1A (-/-) HCT116; HCT116 p21-/-; 80S14
Accession CVCL_HD73
Resource Identification Initiative To cite this cell line use: HCT 116 CDKN1A(-/-) (RRID:CVCL_HD73)
Comments Population: Caucasian.
Knockout cell: Method=Homologous recombination; HGNC; 1784; CDKN1A.
Caution: There are two clones of p21-/- cells that were created, 80S4 and 80S14. It is not clear which clone this cell line corresponds to, but as 80S14 was much more used than 80S4 we believe it is that clone.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 3373; EP300; Simple; p.Asn1700Thrfs*9 (c.5099delA); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 9277; PPM1D; Simple; p.Leu450Ter (c.1349delT) (p.Leu450fs) (c.1344delT); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0291 (HCT 116)
Sex of cell Male
Age at sampling 48Y
Category Cancer cell line
Publications

PubMed=7585571
Waldman T., Kinzler K.W., Vogelstein B.
p21 is necessary for the p53-mediated G1 arrest in human cancer cells.
Cancer Res. 55:5187-5190(1995)

PubMed=8756351; DOI=10.1101/gad.10.15.1945
Polyak K., Waldman T., He T.-C., Kinzler K.W., Vogelstein B.
Genetic determinants of p53-induced apoptosis and growth arrest.
Genes Dev. 10:1945-1952(1996)

Cross-references
Cell line collections (Providers) Horizon_Discovery; HD+R02-035
Cell line databases/resources cancercelllines; CVCL_HD73
Anatomy/cell type resources BTO; BTO:0006532
Encyclopedic resources Wikidata; Q54881926
Entry history
Entry creation01-Dec-2016
Last entry update05-Oct-2023
Version number12