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Cellosaurus RKO BRAF(+/-/-) (CVCL_HE15)

[Text version]

Cell line name RKO BRAF(+/-/-)
Synonyms BRAF (+/-/-) RKO
Accession CVCL_HE15
Resource Identification Initiative To cite this cell line use: RKO BRAF(+/-/-) (RRID:CVCL_HE15)
Comments Characteristics: RKO carries a stable triplication of the BRAF gene with one wild-type (V-600) and two mutant alleles (E-600). This KO cell line carries the wild-type allele.
Knockout cell: Method=Homologous recombination (2 of 3 alleles); HGNC; 1097; BRAF.
Sequence variations Heterozygous for ACVR2A p.Lys437fs*19 (c.1309_1310delAA) and p.Lys437fs*5 (c.1310delA) (from parent cell line).
PIK3CA p.His1047Arg (c.3140A>G) (ClinVar=VCV000013652) (from parent cell line).
Heterozygous for TGFBR2 p.Lys128Serfs*35 (c.383delA) and p.Leu452Pro (c.1355T>C) (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0504 (RKO)
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Cancer cell line
Web pages https://www.horizondiscovery.com/braf-rko-cell-line-hd-106-003
Publications

PubMed=19661383; DOI=10.1126/science.1174229
Yun J., Rago C., Cheong I., Pagliarini R., Angenendt P., Rajagopalan H., Schmidt K., Willson J.K.V., Markowitz S.D., Zhou S., Diaz L.A. Jr., Velculescu V.E., Lengauer C., Kinzler K.W., Vogelstein B., Papadopoulos N.
Glucose deprivation contributes to the development of KRAS pathway mutations in tumor cells.
Science 325:1555-1559(2009)

Cross-references
Other Wikidata; Q54950539
Entry history
Entry creation01-Dec-2016
Last entry update02-Jul-2020
Version number6