ID   RKO BRAF(V600E/-/-)
AC   CVCL_HE17
SY   BRAF (V600E/-/-) RKO
DR   cancercelllines; CVCL_HE17
DR   Horizon_Discovery; HD+106-004
DR   Wikidata; Q54950541
RX   PubMed=19661383;
CC   Characteristics: RKO carries a stable triplication of the BRAF gene with one wild-type (V-600) and two mutant alleles (E-600). This KO cell line carries only the mutant allele.
CC   Knockout cell: Method=Homologous recombination; HGNC; 1097; BRAF (Note=2 of 3 alleles).
CC   Sequence variation: Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1097; BRAF; Simple_edited; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Homozygous; Note=By method not specified (Horizon_Discovery=HD+106-004).
CC   Sequence variation: Mutation; HGNC; 8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11773; TGFBR2; Simple; p.Leu452Pro (c.1355T>C); ClinVar=VCV000930524; Zygosity=Heterozygous (from parent cell line).
CC   Derived from site: In situ; Colon; UBERON=UBERON_0001155.
DI   NCIt; C4910; Colon carcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0504 ! RKO
SX   Sex unspecified
AG   Age unspecified
CA   Cancer cell line
DT   Created: 01-12-16; Last updated: 30-01-24; Version: 12
//
RX   PubMed=19661383; DOI=10.1126/science.1174229;
RA   Yun J., Rago C., Cheong I., Pagliarini R., Angenendt P.,
RA   Rajagopalan H., Schmidt K., Willson J.K.V., Markowitz S.D., Zhou S.-B.,
RA   Diaz L.A. Jr., Velculescu V.E., Lengauer C., Kinzler K.W.,
RA   Vogelstein B., Papadopoulos N.;
RT   "Glucose deprivation contributes to the development of KRAS pathway
RT   mutations in tumor cells.";
RL   Science 325:1555-1559(2009).
//