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Cellosaurus HEK293-EBNA1-6E (CVCL_HF20)

[Text version]

Cell line name HEK293-EBNA1-6E
Synonyms HEK 293EBNA1-6E; HEK293-6E; HEK2936E; 293-6E; 293FEt-clone 6E
Accession CVCL_HF20
Resource Identification Initiative To cite this cell line use: HEK293-EBNA1-6E (RRID:CVCL_HF20)
Comments Group: Patented cell line.
Registration: International Depositary Authority, IDA of Canada (IDAC); 150305-01.
Characteristics: The use of recombinant protein expression vectors containing the EBV oriP in cell lines stably expressing EBV's EBNA1 protein significantly increases recombinant protein yield.
Characteristics: This cell line is transfected with EBNA1t, a truncated version of EBV EBNA1 lacking the Gly-Gly-Ala repeats region. It has an enhanced ability to produce recombinant protein compared to HEK293-EBNA (CVCL_6974).
Transfected with: UniProtKB; P03211; EBV EBNA1 (with del 101-324 = EBNA1t).
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from sampling site: Fetal kidney.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_6642 (HEK293-F)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Web pages https://web.archive.org/web/20190105224206/http://www.nrc-cnrc.gc.ca/eng/solutions/licensing/10894.html
Publications

PubMed=21356792; DOI=10.1101/pdb.prot4976
Tom R., Bisson L., Durocher Y.
Culture of HEK293-EBNA1 cells for production of recombinant proteins.
CSH Protoc. 2008:Pdb.prot4976-Pdb.prot4976(2008)

Patent=US8551774
Durocher Y.
Expression vectors containing a truncated Epstein Barr nuclear antigen 1 lacking the Gly-Gly-Ala domain for enhanced transient gene expression.
Patent number US8551774, 08-Oct-2013

Cross-references
Encyclopedic resources Wikidata; Q54882446
Entry history
Entry creation01-Dec-2016
Last entry update23-Sep-2021
Version number7