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Cellosaurus HCT 116 EP300 WT (CVCL_HG26)

[Text version]

Cell line name HCT 116 EP300 WT
Synonyms HCT 116 p300 WT
Accession CVCL_HG26
Resource Identification Initiative To cite this cell line use: HCT 116 EP300 WT (RRID:CVCL_HG26)
Comments Characteristics: HCT 116 is hemizygous for EP300. This cell line is a normal derivative of HCT 116 from which a number of clones KO for the EP300 single allele were established.
Derived from sampling site: Colon.
Sequence variations Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 3373; EP300; Simple; p.Asn1700Thrfs*9 (c.5099delA); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0291 (HCT 116)
Children:
CVCL_HG27 (HCT 116 EP300(-) clone D10)CVCL_HG28 (HCT 116 EP300(-) clone F2)CVCL_HG29 (HCT 116 EP300(-) clone F5)
Sex of cell Male
Age at sampling 48Y
Category Cancer cell line
STR profile Source(s): ECACC

Markers:
AmelogeninX
CSF1PO7,10
D3S135812.1,18.1
D5S81810,11
D7S82011,12
D8S117910.1,14.1
D13S31710,13
D16S53911,13
D18S5116,17
D21S1129,30
FGA18,23
Penta D9,13
Penta E13,14
TH018,9
TPOX8
vWA17,22

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Publications

PubMed=15123817; DOI=10.1073/pnas.0401002101
Iyer N.G., Chin S.-F., Ozdag H., Daigo Y., Hu D.-E., Cariati M., Brindle K., Aparicio S., Caldas C.
p300 regulates p53-dependent apoptosis after DNA damage in colorectal cancer cells by modulation of PUMA/p21 levels.
Proc. Natl. Acad. Sci. U.S.A. 101:7386-7391(2004)

Cross-references
Cell line collections ECACC; 17011214
Ximbio; 153183
Other Wikidata; Q54881933
Entry history
Entry creation01-Dec-2016
Last entry update20-May-2021
Version number10