ID   HCT 116 EP300(-) clone F2
AC   CVCL_HG28
SY   HCT 116 p300 KO Clone F2; HCT 116 p300 KO [F2]; p300 KO Clone F2
DR   ECACC; 17011215
DR   Wikidata; Q54881935
DR   Ximbio; 153185
RX   PubMed=15123817;
CC   Characteristics: HCT 116 is hemizygous for EP300. This cell line is KO for the single allele.
CC   Knockout cell: Method=Homologous recombination; HGNC; 3373; EP300.
CC   Sequence variation: Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 3373; EP300; Simple; p.Asn1700Thrfs*9 (c.5099delA); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
CC   Derived from sampling site: Colon.
ST   Source(s): ECACC
ST   Amelogenin: X
ST   CSF1PO: 7,10
ST   D13S317: 10,13
ST   D16S539: 11,13.1
ST   D18S51: 16,17
ST   D21S11: 29,30
ST   D3S1358: 12,17
ST   D5S818: 10,11
ST   D7S820: 11,12
ST   D8S1179: 11,12
ST   FGA: 18,23
ST   Penta D: 9,13
ST   Penta E: 13,14
ST   TH01: 8,9
ST   TPOX: 8
ST   vWA: 18,21
DI   NCIt; C4910; Colon carcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens
HI   CVCL_HG26 ! HCT 116 EP300 WT
SX   Male
AG   48Y
CA   Cancer cell line
DT   Created: 01-12-16; Last updated: 20-05-21; Version: 11
//
RX   PubMed=15123817; DOI=10.1073/pnas.0401002101;
RA   Iyer N.G., Chin S.-F., Ozdag H., Daigo Y., Hu D.-E., Cariati M.,
RA   Brindle K., Aparicio S., Caldas C.;
RT   "p300 regulates p53-dependent apoptosis after DNA damage in colorectal
RT   cancer cells by modulation of PUMA/p21 levels.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7386-7391(2004).
//