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Cellosaurus CHO-K1 tsTM4 (CVCL_J617)

[Text version]
Cell line name CHO-K1 tsTM4
Synonyms tsTM4
Accession CVCL_J617
Resource Identification Initiative To cite this cell line use: CHO-K1 tsTM4 (RRID:CVCL_J617)
Comments Characteristics: Temperature sensitive. Exhibits abnormal induction of sister chromatid exchanges along with a decrease of DNA synthesis in the cells arrested in S phase at the non-permissive temperature of 39 Celsius (PubMed=9272869).
Doubling time: 34 hours (PubMed=2237640).
Transformant: ChEBI; CHEBI:21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Mutation; UniProtKB; P11414; Polr2a; Simple; p.Pro1006Ser (c.3016C>T); Zygosity=Homozygous (PubMed=9272869).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_0214 (CHO-K1)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=2237640; DOI=10.1007/BF01233196
Tsuji H., Matsudo Y., Tsuji S., Hanaoka F., Hyodo M., Hori T.-a.
Isolation of temperature-sensitive CHO-K1 cell mutants exhibiting chromosomal instability and reduced DNA synthesis at nonpermissive temperature.
Somat. Cell Mol. Genet. 16:461-476(1990)

PubMed=9272869; DOI=10.1016/S0378-1119(97)00204-7
Sugaya K., Sasanuma S.-i., Nohata J., Kimura T., Hongo E., Higashi T., Morimyo M., Tsuji H., Mita K.
Cloning and sequencing for the largest subunit of Chinese hamster RNA polymerase II gene: identification of a mutation related to abnormal induction of sister chromatid exchanges.
Gene 194:267-272(1997)

Cross-references
Encyclopedic resources Wikidata; Q54973166
Entry history
Entry creation11-Feb-2013
Last entry update30-Jan-2024
Version number10