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Cellosaurus A-375 NRAS p.Q61K (CVCL_JF22)

Cell line name A-375 NRAS p.Q61K
Synonyms A-375 NRASQ61K; NRAS mutant-A375 Isogenic Cell Line
Accession CVCL_JF22
Resource Identification Initiative To cite this cell line use: A-375 NRAS p.Q61K (RRID:CVCL_JF22)
Comments Characteristics: Shows significant resistance to the BRAF inhibitors Dabrafineb and Vemurafenib when compared to its parental cell line in 2D and 3D culture systems. Can be a useful model to study the RAS-RAF-MEK-ERK-MAP kinase signaling pathway and to screen potential BRAF inhibitors and anti-cancer compounds for drug discovery and development (ATCC).
Sequence variations Homozygous for NRAS p.Gln61Lys (c.181C>A); by CRISPR/Cas9 (ATCC).
Homozygous for BRAF p.Val600Glu (c.1799T>A) (from parent cell line).
Homozygous for CDKN2A p.Glu61Ter (c.181G>T) (p.Gly75Val; c.224G>T) and p.Glu69Ter (c.205G>T) (p.Gly83Val; c.248G>T) (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0132 (A-375)
Sex of cell Female
Age at sampling 54Y
Category Cancer cell line
STR profile Source(s): ATCC

Markers:
AmelogeninX
CSF1PO11,12
D13S31711,14
D16S5399
D5S81812
D7S8209
TH018
TPOX8,10
vWA16,17
Web pages https://www.atcc.org/support/faqs/874a3/Application%20of%20Isogenic%20cell%20line%20with%20RAS%20mutation.aspx
Cross-references
Cell line collections ATCC; CRL-1619IG-2
Other Wikidata; Q54605989