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Cellosaurus RT4-67 (CVCL_JK04)

[Text version]
Cell line name RT4-67
Synonyms RT4-D6P2T clone 67
Accession CVCL_JK04
Resource Identification Initiative To cite this cell line use: RT4-67 (RRID:CVCL_JK04)
Comments Characteristics: Expression of NF2 is doxycycline-inducible as the parent cell line was transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 (PubMed=11316791).
Transfected with: HGNC; 7773; NF2.
Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Transformant: ChEBI; CHEBI:23995; N-ethyl-N-nitrosourea (ENU).
Derived from site: In situ; Peripheral nervous system, sciatic nerve; UBERON=UBERON_0001322.
Cell type: Schwann cell; CL=CL_0002573.
Disease Rat malignant schwannoma (NCIt: C60465)
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: BDIX.
Hierarchy Parent: CVCL_4006 (RT4-D6P2T)
Category Cancer cell line
Publications

PubMed=11316791; DOI=10.1101/gad.189601
Morrison H., Sherman L.S., Legg J., Banine F., Isacke C., Haipek C.A., Gutmann D.H., Ponta H., Herrlich P.
The NF2 tumor suppressor gene product, merlin, mediates contact inhibition of growth through interactions with CD44.
Genes Dev. 15:968-980(2001)

PubMed=14580336; DOI=10.1016/S1097-2765(03)00382-4
Kissil J.L., Wilker E.W., Johnson K.C., Eckman M.S., Yaffe M.B., Jacks T.
Merlin, the product of the Nf2 tumor suppressor gene, is an inhibitor of the p21-activated kinase, Pak1.
Mol. Cell 12:841-849(2003)

PubMed=21481793; DOI=10.1016/j.ccr.2011.02.017
Yi C.-L., Troutman S., Fera D., Stemmer-Rachamimov A.O., Avila J.L., Christian N., Persson N.L., Shimono A., Speicher D.W., Marmorstein R., Holmgren L., Kissil J.L.
A tight junction-associated merlin-angiomotin complex mediates merlin's regulation of mitogenic signaling and tumor suppressive functions.
Cancer Cell 19:527-540(2011)

PubMed=28469964
Guo J.-M., Grovola M.R., Xie H., Coggins G.E., Duggan P., Hasan R., Huang J.-L., Lin D.W., Song C., Witek G.M., Berritt S., Schultz D.C., Field J.
Comprehensive pharmacological profiling of neurofibromatosis cell lines.
Am. J. Cancer Res. 7:923-934(2017)

Cross-references
Encyclopedic resources Wikidata; Q54951361
Entry history
Entry creation15-May-2017
Last entry update30-Jan-2024
Version number8