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Cellosaurus Lec13 (CVCL_KA69)

[Text version]
Cell line name Lec13
Synonyms CHO-Lec13; Pro-Lec13.6a
Accession CVCL_KA69
Resource Identification Initiative To cite this cell line use: Lec13 (RRID:CVCL_KA69)
Comments Characteristics: Useful for the production of fucose-negative antibodies.
Characteristics: Deficient in GDP-mannose 4,6 dehydratase (GMDS).
Selected for resistance to: UniProtKB; P02867; Pisum sativum lectin.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_4382 (CHO Pro-5)
Children:
CVCL_D2TR (Lec13/PODXL)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=2428310; DOI=10.1016/0003-9861(86)90031-7
Ripka J., Adamany A., Stanley P.
Two Chinese hamster ovary glycosylation mutants affected in the conversion of GDP-mannose to GDP-fucose.
Arch. Biochem. Biophys. 249:533-545(1986)

PubMed=3456174; DOI=10.1007/BF01560727
Ripka J., Stanley P.
Lectin-resistant CHO cells: selection of four new pea lectin-resistant phenotypes.
Somat. Cell Mol. Genet. 12:51-62(1986)

PubMed=11986321; DOI=10.1074/jbc.M202069200
Shields R.L., Lai J., Keck R., O'Connell L.Y., Hong K., Meng Y.G., Weikert S.H.A., Presta L.G.
Lack of fucose on human IgG1 N-linked oligosaccharide improves binding to human Fcgamma RIII and antibody-dependent cellular toxicity.
J. Biol. Chem. 277:26733-26740(2002)

PubMed=16609957; DOI=10.1002/bit.20880
Kanda Y., Yamane-Ohnuki N., Sakai N., Yamano K., Nakano R., Inoue-Urakubo M., Misaka H., Iida S., Wakitani M., Konno Y., Yano K., Shitara K., Hosoi S., Satoh M.
Comparison of cell lines for stable production of fucose-negative antibodies with enhanced ADCC.
Biotechnol. Bioeng. 94:680-688(2006)

Cross-references
Encyclopedic resources Wikidata; Q54902419
Entry history
Entry creation22-Aug-2017
Last entry update30-Jan-2024
Version number7