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Cellosaurus PathHunter THP-1 IkappaB Degradation (CVCL_KZ70)

[Text version]

Cell line name PathHunter THP-1 IkappaB Degradation
Accession CVCL_KZ70
Resource Identification Initiative To cite this cell line use: PathHunter THP-1 IkappaB Degradation (RRID:CVCL_KZ70)
Comments Population: Japanese.
Characteristics: PathHunter degradation cell lines express a target protein tagged with a beta-galactosidase fragment (Enzyme Donor=ED). Pathway activation results in the degradation of the target protein. The degraded protein is detected by adding to the cell lysis buffer another beta-galactosidase fragment (Enzyme Acceptor=EA). Complementation of the ED and EA enzyme fragments result into a catalytically active enzyme that generate a chemiluminescent signal.
Transfected with: HGNC; 7797; NFKBIA.
Derived from sampling site: Peripheral blood.
Sequence variations Gene fusion; HGNC; 2457; CSNK2A1 + HGNC; 13917; DDX39B; Name(s)=CSNK2A1-DDX39B (from parent cell line).
Gene fusion; HGNC; 7132; KMT2A + HGNC; 7136; MLLT3; Name(s)=KMT2A-MLLT3, MLL-MLLT3, MLL-AF9 (from parent cell line).
Mutation; HGNC; 7989; NRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000039648; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 11998; TP53; Simple; p.Arg174fs*3 (c.520_545del26); Zygosity=Heterozygous (from parent cell line).
Disease Childhood acute monocytic leukemia (NCIt: C9163)
Acute monoblastic/monocytic leukemia (ORDO: Orphanet_514)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0006 (THP-1)
Sex of cell Male
Age at sampling 1Y
Category Cancer cell line
Cross-references
Cell line collections DiscoverX; 93-0538C14
Other Wikidata; Q54938064
Entry history
Entry creation22-Aug-2017
Last entry update20-May-2021
Version number8