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Cellosaurus PathHunter U2OS CDC25A Degradation (CVCL_KZ99)

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Cell line name PathHunter U2OS CDC25A Degradation
Accession CVCL_KZ99
Resource Identification Initiative To cite this cell line use: PathHunter U2OS CDC25A Degradation (RRID:CVCL_KZ99)
Comments Characteristics: PathHunter degradation cell lines express a target protein tagged with a beta-galactosidase fragment (Enzyme Donor=ED). Pathway activation results in the degradation of the target protein. The degraded protein is detected by adding to the cell lysis buffer another beta-galactosidase fragment (Enzyme Acceptor=EA). Complementation of the ED and EA enzyme fragments result into a catalytically active enzyme that generate a chemiluminescent signal.
Transfected with: HGNC; 1725; CDC25A.
Derived from sampling site: Bone; tibia.
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Cross-references
Cell line collections DiscoverX; 93-0765C3
Other Wikidata; Q54938108
Entry history
Entry creation22-Aug-2017
Last entry update02-Jul-2020
Version number5