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Cellosaurus KM20 (CVCL_L095)

[Text version]
Cell line name KM20
Synonyms KM-20
Accession CVCL_L095
Resource Identification Initiative To cite this cell line use: KM20 (RRID:CVCL_L095)
Comments Problematic cell line: Contaminated. Shown to be a HT-29 derivative (ICLAC; an early sample of KM20 had been tested by the core facility where the cell line was established and the STR profile corresponds to that of HT-29).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00513.
Population: Caucasian.
Microsatellite instability: Stable (MSS) (PubMed=25926053).
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; 583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0320 (HT-29)
Children:
CVCL_0B38 (KM20-C)
Sex of cell Female
Age at sampling 44Y
Category Cancer cell line
STR profile Source(s): PubMed=25926053

Markers:
AmelogeninX
CSF1PO11,12
D5S81811,12
D7S82010
D13S31711
D16S53911,12
D21S1129,30
TH016
TPOX8,9
vWA17,19

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
Publications

PubMed=2846163
Morikawa K., Walker S.M., Nakajima M., Pathak S., Jessup J.M., Fidler I.J.
Influence of organ environment on the growth, selection, and metastasis of human colon carcinoma cells in nude mice.
Cancer Res. 48:6863-6871(1988)

PubMed=12060639
Wang Q.-D., Li N., Wang X.-F., Kim M.M., Evers B.M.
Augmentation of sodium butyrate-induced apoptosis by phosphatidylinositol 3'-kinase inhibition in the KM20 human colon cancer cell line.
Clin. Cancer Res. 8:1940-1947(2002)

PubMed=25926053; DOI=10.1038/ncomms8002
Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G., Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S., Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M., Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002.1-7002.10(2015)

Cross-references
Cell line collections (Providers) KCLB; 80017 - Discontinued
Cell line databases/resources cancercelllines; CVCL_L095
Biological sample resources BioSample; SAMN06481114
Encyclopedic resources Wikidata; Q54900064
Gene expression databases GEO; GSM1448074
Entry history
Entry creation06-May-2013
Last entry update05-Oct-2023
Version number21