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Cellosaurus NAKT-15 (CVCL_L910)

[Text version]
Cell line name NAKT-15
Synonyms NAKT15
Accession CVCL_L910
Resource Identification Initiative To cite this cell line use: NAKT-15 (RRID:CVCL_L910)
Comments Characteristics: Secretes insulin in response to glucose stimulation and nonglucose secretagogues, and expresses proteins characteristic of beta-cells.
Characteristics: Because it has been transduced by two constructs containing LoxP sites, transfection with the Cre recombinase eliminates both the SV40 T gene and TERT thus reverting the cells to a non-immortal phenotype.
Characteristics: Transduced with two retroviral vectors: 1) SSR#197 (MoMLV/LTR-LoxP-hTERT-IRES-EGFP-LoxP-MoMLV/LTR) expressing hTERT and eGFP flanked by two loxP sites and 2) SSR#69 (MoMLV/LTR-LoxP-HygroR-HSV/TK-IRES-SV40T-LoxP-NeoR-MoMLV/LTR) expressing HygroR, HSV-1 TK and SV40 T-antigen flanked by two loxP sites.
Transfected with: HGNC; 11730; TERT.
Transfected with: UniProtKB; Q9QNF7; Human herpesvirus 1 TK.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Pancreas, islets of Langerhans; UBERON=UBERON_0000006.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Transformed cell line
Publications

PubMed=16186810; DOI=10.1038/nbt1145
Narushima M., Kobayashi N., Okitsu T., Tanaka Y., Li S.-A., Chen Y., Miki A., Tanaka K., Nakaji S., Takei K., Gutierrez A.S., Rivas-Carrillo J.D., Navarro-Alvarez N., Jun H.-S., Westerman K.A., Noguchi H., Lakey J.R.T., Leboulch P., Tanaka N., Yoon J.-W.
A human beta-cell line for transplantation therapy to control type 1 diabetes.
Nat. Biotechnol. 23:1274-1282(2005)

PubMed=16211061; DOI=10.1038/nbt1005-1231
Hohmeier H.-E., Newgard C.B.
Islets for all?
Nat. Biotechnol. 23:1231-1232(2005)

Cross-references
Encyclopedic resources Wikidata; Q54907375
Entry history
Entry creation06-May-2013
Last entry update05-Oct-2023
Version number12