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Cellosaurus PathHunter U2OS F2RL1 Activated GPCR Internalization (CVCL_LA27)

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Cell line name PathHunter U2OS F2RL1 Activated GPCR Internalization
Synonyms PathHunter F2RL1 Activated GPCR Internalization U2OS
Accession CVCL_LA27
Resource Identification Initiative To cite this cell line use: PathHunter U2OS F2RL1 Activated GPCR Internalization (RRID:CVCL_LA27)
Comments Characteristics: PathHunter activated GPCR internalization cell lines co-express an untagged GPCR, a beta-arrestin tagged with a beta-galactosidase fragment (Enzyme Acceptor=EA) and a second beta-galactosidase fragment (ProLink=PK) localized to the endosomes. Beta-arrestin recruitment is followed by internalization of the GPCR/arrestin-EA complex in PK-tagged endosomes and allowing complementation of the PK and EA enzyme fragments thus resulting into a catalytically active enzyme that generate a chemiluminescent signal.
Transfected with: HGNC; 3538; F2RL1.
Transfected with: HGNC; 712; ARRB2.
Derived from sampling site: Bone; tibia.
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Cross-references
Cell line collections DiscoverX; 93-0747C3
Other Wikidata; Q54938141
Entry history
Entry creation22-Aug-2017
Last entry update12-Mar-2020
Version number4