ID   PathHunter U2OS GPR35 Activated GPCR Internalization
AC   CVCL_LA43
SY   PathHunter GPR35 Activated GPCR Internalization U2OS
DR   DiscoverX; 93-0968C3
DR   Wikidata; Q54938168
CC   Characteristics: PathHunter activated GPCR internalization cell lines co-express an untagged GPCR, a beta-arrestin tagged with a beta-galactosidase fragment (Enzyme Acceptor=EA) and a second beta-galactosidase fragment (ProLink=PK) localized to the endosomes. Beta-arrestin recruitment is followed by internalization of the GPCR/arrestin-EA complex in PK-tagged endosomes and allowing complementation of the PK and EA enzyme fragments thus resulting into a catalytically active enzyme that generate a chemiluminescent signal.
CC   Transfected with: HGNC; 4492; GPR35.
CC   Transfected with: HGNC; 712; ARRB2.
CC   Derived from sampling site: Bone; tibia.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 22-08-17; Last updated: 12-03-20; Version: 4
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