ID   PathHunter U2OS TACR1 Activated GPCR Internalization
SY   PathHunter TACR1 Activated GPCR Internalization U2OS
DR   DiscoverX; 93-0717C3
DR   Wikidata; Q54938296
CC   Population: Caucasian.
CC   Characteristics: PathHunter activated GPCR internalization cell lines co-express an untagged GPCR, a beta-arrestin tagged with a beta-galactosidase fragment (Enzyme Acceptor=EA) and a second beta-galactosidase fragment (ProLink=PK) localized to the endosomes. Beta-arrestin recruitment is followed by internalization of the GPCR/arrestin-EA complex in PK-tagged endosomes and allowing complementation of the PK and EA enzyme fragments thus resulting into a catalytically active enzyme that generate a chemiluminescent signal.
CC   Transfected with: HGNC; 11526; TACR1.
CC   Transfected with: HGNC; 712; ARRB2.
CC   Derived from sampling site: Bone; tibia.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 22-08-17; Last updated: 12-01-21; Version: 5