Home  |  Contact

Cellosaurus Jurkat 1F2 (CVCL_LH47)

[Text version]

Cell line name Jurkat 1F2
Synonyms 1F2
Accession CVCL_LH47
Resource Identification Initiative To cite this cell line use: Jurkat 1F2 (RRID:CVCL_LH47)
Comments Characteristics: One of the IL2 allele was inactived by CRISPR/Cas9 and a luciferase gene was inserted in the loci thus expressing the luciferase gene under the control of endogenous IL2 promoter.
Knockout cell: Method=CRISPR/Cas9 (1 of 2 alleles); HGNC; 6001; IL2.
Transfected with: UniProtKB; P08659; Firefly luciferase.
Derived from sampling site: Peripheral blood.
Sequence variations Heterozygous for BAX p.Glu41Argfs*19 (c.121delG) (ClinVar=VCV000009512) (from parent cell line).
Heterozygous for BAX p.Glu41Glyfs*33 (c.121dupG) (ClinVar=VCV000009511) (from parent cell line).
Heterozygous for FBXW7 p.Arg505Cys (c.1513C>T) (ClinVar=VCV000069961) (from parent cell line).
Heterozygous for INPP5D p.Gln345Ter (c.1033C>T) (from parent cell line).
Heterozygous for INPP5D c.1097+1065_1097+1112del47 (from parent cell line).
Homozygous for MSH2 p.Arg711Ter (c.2131C>T) (ClinVar=VCV000090903) (from parent cell line).
Homozygous for MSH6 p.Phe1088Serfs*2 (c.3261delC) (ClinVar=VCV000089363) (from parent cell line).
Heterozygous for SYK p.Met34Hisfs*3 (c.98_99insG) (from parent cell line).
Heterozygous for TP53 p.Arg196Ter (c.586C>T) (ClinVar=VCV000043589) (from parent cell line).
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0367 (Jurkat E6.1)
Children:
CVCL_LH48 (Jurkat 1F2-hGITR)
Sex of cell Male
Age at sampling 14Y
Category Cancer cell line
Publications

PubMed=28847736; DOI=10.1016/j.jim.2017.08.006
Liu J.-Q., Liu R., Gray P., Liu Z.-Y., Cui X.-X., Li G.-H., Liu Z.
Development of a luciferase reporter Jurkat cell line under the control of endogenous interleukin-2 promoter.
J. Immunol. Methods 451:48-53(2017)

Cross-references
Other Wikidata; Q54899126
Entry history
Entry creation15-Nov-2017
Last entry update12-Jan-2021
Version number9