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Cellosaurus CHO-K1 tsTM13 (CVCL_LH73)

[Text version]
Cell line name CHO-K1 tsTM13
Synonyms tsTM13
Accession CVCL_LH73
Resource Identification Initiative To cite this cell line use: CHO-K1 tsTM13 (RRID:CVCL_LH73)
Comments Characteristics: Temperature sensitive. Exhibits a delayed cell cycle progression from metaphase to telophase at a non-permissive temperature of 39 Celsius and is finally arrested from anaphase to telophase.
Transformant: ChEBI; CHEBI:21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_0214 (CHO-K1)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=2237640; DOI=10.1007/BF01233196
Tsuji H., Matsudo Y., Tsuji S., Hanaoka F., Hyodo M., Hori T.-a.
Isolation of temperature-sensitive CHO-K1 cell mutants exhibiting chromosomal instability and reduced DNA synthesis at nonpermissive temperature.
Somat. Cell Mol. Genet. 16:461-476(1990)

PubMed=1309502; DOI=10.1016/0014-4827(92)90372-F
Tsuji H., Matsudo Y., Ajiro K., Yasuda H., Hanaoka F., Hayashi A., Utsumi S., Ohba Y., Hori T.-a.
A temperature-sensitive CHO-K1 cell mutant (tsTM13) defective in chromosome decondensation and spindle deconstruction in M phase.
Exp. Cell Res. 198:201-213(1992)

PubMed=8944784; DOI=10.1111/j.1432-1033.1996.00923.x
Ajiro K., Yasuda H., Tsuji H.
Vanadate triggers the transition from chromosome condensation to decondensation in a mitotic mutant (tsTM13) inactivation of p34cdc2/H1 kinase and dephosphorylation of mitosis-specific histone H3.
Eur. J. Biochem. 241:923-930(1996)

Cross-references
Encyclopedic resources Wikidata; Q54973161
Entry history
Entry creation15-Nov-2017
Last entry update30-Jan-2024
Version number8