Home  |  Contact

Cellosaurus A549 VIM RFP (CVCL_LI35)

[Text version]
Cell line name A549 VIM RFP
Synonyms A549 Vim RFP; A-549 VIM RFP
Accession CVCL_LI35
Resource Identification Initiative To cite this cell line use: A549 VIM RFP (RRID:CVCL_LI35)
Comments Population: Caucasian.
Characteristics: Using CRISPR/Cas9 a red fluorescent protein (RFP) tag was added to the C-terminal of the VIM gene. Allows to monitor the epithelial-to-mesenchymal transition (EMT) status of cells in vitro by monitoring RFP expression.
Transfected with: UniProtKB; Q9U6Y8; Discosoma sp. red fluorescent protein drFP583 (DsRed, RFP).
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0023 (A-549)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
STR profile Source(s): ATCC

Markers:
AmelogeninX,Y
CSF1PO10,12
D5S81811
D7S8208,11
D13S31711
D16S53911,12
TH018,9.3
TPOX8,11
vWA14

Run an STR similarity search on this cell line
Cross-references
Cell line collections (Providers) ATCC; CCL-185EMT
Cell line databases/resources cancercelllines; CVCL_LI35
Encyclopedic resources Wikidata; Q54607012
Entry history
Entry creation15-Nov-2017
Last entry update05-Oct-2023
Version number11