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Cellosaurus UHi006-A-2 (CVCL_LJ88)

[Text version]

Cell line name UHi006-A-2
Synonyms HEL24.3-OCT4-nEmGFP; HEL24.3-OCT4-nEmGFP-A-G3; UHi001-B
Accession CVCL_LJ88
Resource Identification Initiative To cite this cell line use: UHi006-A-2 (RRID:CVCL_LJ88)
Comments From: University of Helsinki; Helsinki; Finland.
Characteristics: Expresses bright nuclear located EmGFP upon POU5F1 activation. Heterozygous knockin of a T2A-NLS-EmGFP-P2A-Pac cassette at the C-terminus of the POU5F1/OCT4 gene by CRISPR/Cas9.
Transfected with: UniProtKB; P42212; GFP (with p.Ser65Thr, p.Ser72Ala, p.Asn149Lys, p.Met153Thr, p.Ile167Thr and p.His231Leu = EmGFP).
Transfected with: UniProtKB; P13249; S.alboniger pac.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9T96 (UHi006-A)
Sex of cell Male
Age at sampling <1M
Category Induced pluripotent stem cell
STR profile Source(s): PubMed=28925359

Markers:
AmelogeninX,Y
CSF1PO10
D5S81811,12
D7S8208,10
D13S31712
D16S53911,12
D21S1129
TH017,9.3
TPOX11
vWA16,18

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Publications

PubMed=28925359; DOI=10.1016/j.scr.2017.07.006
Balboa D., Weltner J., Novik Y., Eurola S., Wartiovaara K., Otonkoski T.
Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9.
Stem Cell Res. 23:105-108(2017)

Cross-references
Cell line databases/resources hPSCreg; UHi006-A-2
Biological sample resources BioSamples; SAMEA104277230
Other Wikidata; Q54990148
Entry history
Entry creation15-Nov-2017
Last entry updated05-Jul-2019
Version number5