ID   UHi006-A-2
AC   CVCL_LJ88
SY   HEL24.3-OCT4-nEmGFP; HEL24.3-OCT4-nEmGFP-A-G3; UHi001-B
DR   BioSamples; SAMEA104277230
DR   hPSCreg; UHi006-A-2
DR   Wikidata; Q54990148
RX   PubMed=28925359;
CC   From: University of Helsinki; Helsinki; Finland.
CC   Population: Caucasian.
CC   Characteristics: Expresses bright nuclear located EmGFP upon POU5F1 activation. Heterozygous knockin of a T2A-NLS-EmGFP-P2A-Pac cassette at the C-terminus of the POU5F1/OCT4 gene by CRISPR/Cas9.
CC   Transfected with: UniProtKB; P42212; GFP (with p.Ser65Thr, p.Ser72Ala, p.Asn149Lys, p.Met153Thr, p.Ile167Thr and p.His231Leu = EmGFP).
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
CC   Cell type: Fibroblast of foreskin; CL=CL_1001608.
ST   Source(s): PubMed=28925359
ST   Amelogenin: X,Y
ST   CSF1PO: 10
ST   D13S317: 12
ST   D16S539: 11,12
ST   D21S11: 29
ST   D5S818: 11,12
ST   D7S820: 8,10
ST   TH01: 7,9.3
ST   TPOX: 11
ST   vWA: 16,18
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9T96 ! UHi006-A
SX   Male
AG   <1M
CA   Induced pluripotent stem cell
DT   Created: 15-11-17; Last updated: 29-06-23; Version: 10
//
RX   PubMed=28925359; DOI=10.1016/j.scr.2017.07.006;
RA   Balboa D., Weltner J., Novik Y., Eurola S., Wartiovaara K.,
RA   Otonkoski T.;
RT   "Generation of an OCT4 reporter human induced pluripotent stem cell
RT   line using CRISPR/SpCas9.";
RL   Stem Cell Res. 23:105-108(2017).
//