Cellosaurus 3AB-OS (CVCL_LM95)

Cell line name 3AB-OS
Accession CVCL_LM95
Resource Identification Initiative To cite this cell line use: 3AB-OS (RRID:CVCL_LM95)
Comments Group: Cancer stem cell line.
Problematic cell line: STR profile provided by Millipore differs greatly from that of the parent MG-63 cell line.
Characteristics: Has cancer stem cell-like characteristics. Produced by long term treatment of MG-63 with 3-aminobenzamide (3AB), an inhibitor of poly(ADP-ribose) polymerase.
Doubling time: ~22 hours (PubMed=19160414).
Selected for resistance to: ChEBI; CHEBI:64042; 3-aminobenzamide (3AB).
Omics: Array-based CGH.
Omics: CNV analysis.
Omics: Transcriptome analysis.
Disease Osteosarcoma (NCIt: C9145)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0426 (MG-63)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): Millipore

Markers:
AmelogeninX
CSF1PO10,11
D13S31713
D16S53910,11
D18S5113,18
D21S1128,29
D3S135815,17
D5S81811
D7S8208,12
D8S117911,14,15
FGA20,21
Penta D11,13
Penta E12
TH019.3
TPOX9
vWA18
Publications

PubMed=19160414; DOI=10.1002/jcp.21667
Di Fiore R., Santulli A., Ferrante R.D., Giuliano M., De Blasio A., Messina C., Pirozzi G., Tirino V., Tesoriere G., Vento R.
Identification and expansion of human osteosarcoma-cancer-stem cells by long-term 3-aminobenzamide treatment.
J. Cell. Physiol. 219:301-313(2009)

DOI=10.4236/scd.2013.33024
Fiore R., Drago-Ferrante R., D'Anneo A., De Blasio A., Santulli A., Messina C., Carlisi D., Tesoriere G., Vento R.
Differentiation of human osteosarcoma 3AB-OS stem-like cells in derivatives of the three primary germ layers as a useful in vitro model to develop several purposes.
Stem Cell Discov. 3:188-201(2013)

PubMed=23129384; DOI=10.1002/jcp.24272
Di Fiore R., Fanale D., Drago-Ferrante R., Chiaradonna F., Giuliano M., De Blasio A., Amodeo V., Corsini L.R., Bazan V., Tesoriere G., Vento R., Russo A.
Genetic and molecular characterization of the human osteosarcoma 3AB-OS cancer stem cell line: a possible model for studying osteosarcoma origin and stemness.
J. Cell. Physiol. 228:1189-1201(2013)

DOI=10.14800/ccm.134
Di Fiore R., Drago-Ferrante R., Marcatti M., Carlisi D., Tesoriere G., Vento R.
A short story of 3AB-OS cancer stem cells, a possible model for studying cancer stemness.
Cancer Cell Microenviron. 1:e134-e134(2014)

Cross-references
Cell line collections Millipore; SCC141