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Cellosaurus WI-38/hTERT/GFP-RAF-ER (CVCL_N806)

[Text version]

Cell line name WI-38/hTERT/GFP-RAF-ER
Accession CVCL_N806
Resource Identification Initiative To cite this cell line use: WI-38/hTERT/GFP-RAF-ER (RRID:CVCL_N806)
Comments Part of: ENCODE project common cell types; tier 3.
Characteristics: Transfected with a construct that is composed of EGFP at the N-terminus, the catalytic domain of RAF1 (with 340-DD-341) in the middle and the hormone-binding domain of ESR1 at the C-terminus. Protein kinase activity is activated by estrogen or analogs such as 4-hydroxy-tamoxifen.
Characteristics: Cells can be senesced after exposure for 72 hours with 4-hydroxy-tamoxifen.
Transfected with: HGNC; 9829; RAF1 (catalytic domain with D-340 and D-341).
Transfected with: HGNC; 3467; ESR1 (hormone-binding domain).
Transfected with: HGNC; 11730; TERT.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Omics: H3K4me3 ChIP-seq epigenome analysis.
Derived from sampling site: Fetal lung.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0579 (WI-38)
Sex of cell Female
Age at sampling 16FW
Category Telomerase immortalized cell line
Web pages http://genome.ucsc.edu/ENCODE/protocols/cell/human/WI38_Stam_protocol.pdf
Cross-references
Biological sample resources ENCODE; ENCBS070MZP
ENCODE; ENCBS157XHV
ENCODE; ENCBS601ENJ
ENCODE; ENCBS606ENC
ENCODE; ENCBS607ENC
ENCODE; ENCBS608ENC
ENCODE; ENCBS715PYA
ENCODE; ENCBS787LHJ
Gene expression databases GEO; GSM651580
GEO; GSM651581
GEO; GSM651582
GEO; GSM651583
GEO; GSM945215
GEO; GSM945223
GEO; GSM945245
GEO; GSM945265
Other Wikidata; Q54994036
Entry history
Entry creation05-Nov-2013
Last entry update19-Dec-2019
Version number11