ID   WI-38/hTERT/GFP-RAF-ER
AC   CVCL_N806
DR   ENCODE; ENCBS070MZP
DR   ENCODE; ENCBS157XHV
DR   ENCODE; ENCBS601ENJ
DR   ENCODE; ENCBS606ENC
DR   ENCODE; ENCBS607ENC
DR   ENCODE; ENCBS608ENC
DR   ENCODE; ENCBS715PYA
DR   ENCODE; ENCBS787LHJ
DR   GEO; GSM651580
DR   GEO; GSM651581
DR   GEO; GSM651582
DR   GEO; GSM651583
DR   GEO; GSM945215
DR   GEO; GSM945223
DR   GEO; GSM945245
DR   GEO; GSM945265
DR   Wikidata; Q54994036
WW   http://genome.ucsc.edu/ENCODE/protocols/cell/human/WI38_Stam_protocol.pdf
CC   Part of: ENCODE project common cell types; tier 3.
CC   Characteristics: Transfected with a construct that is composed of EGFP at the N-terminus, the catalytic domain of RAF1 (with 340-DD-341) in the middle and the hormone-binding domain of ESR1 at the C-terminus. Protein kinase activity is activated by estrogen or analogs such as 4-hydroxy-tamoxifen.
CC   Characteristics: Cells can be senesced after exposure for 72 hours with 4-hydroxy-tamoxifen.
CC   Transfected with: HGNC; 9829; RAF1 (catalytic domain with D-340 and D-341).
CC   Transfected with: HGNC; 3467; ESR1 (hormone-binding domain).
CC   Transfected with: HGNC; 11730; TERT.
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Omics: H3K4me3 ChIP-seq epigenome analysis.
CC   Derived from sampling site: Fetal lung.
OX   NCBI_TaxID=9606; ! Homo sapiens
HI   CVCL_0579 ! WI-38
SX   Female
AG   16FW
CA   Telomerase immortalized cell line
DT   Created: 05-11-13; Last updated: 19-12-19; Version: 11
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