Cell line name | 4T1-mNIS-Neo/eGFP-Puro |
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Accession | CVCL_QZ62 |
Resource Identification Initiative | To cite this cell line use: 4T1-mNIS-Neo/eGFP-Puro (RRID:CVCL_QZ62) |
Comments | Characteristics: Reporter cell line, transfected with both the LV-mNIS-P2A-Neo and LV-eGFP-PGK-Puro vectors. LV-mNIS-P2A-Neo encodes for mouse Slc5a5 linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-eGFP-PGK-Puro encodes for eGFP under control of the spleen focus-forming virus (SFFV) promoter and the pac gene under the control of the mouse PGK promoter. Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG). Transfected with: MGI; MGI:2149330; Slc5a5. Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; P00552; Transposon Tn5 neo. Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911. |
Disease | Malignant neoplasms of the mouse mammary gland (NCIt: C21678) |
Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: BALB/cfC3H. |
Hierarchy | Parent: CVCL_0125 (4T1) |
Sex of cell | Female |
Category | Cancer cell line |
Cross-references | |
Cell line collections (Providers) | Imanis; CL025 |
Encyclopedic resources | Wikidata; Q54603546 |
Entry history | |
Entry creation | 05-Mar-2018 |
Last entry update | 05-Oct-2023 |
Version number | 8 |