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Cellosaurus B16F10-Fluc-Neo/mNIS-Puro (CVCL_QZ89)

[Text version]

Cell line name B16F10-Fluc-Neo/mNIS-Puro
Accession CVCL_QZ89
Resource Identification Initiative To cite this cell line use: B16F10-Fluc-Neo/mNIS-Puro (RRID:CVCL_QZ89)
Comments Characteristics: Reporter cell line, transfected with both the LV-Fluc-P2A-Neo and LV-mNIS-PGK-Puro vectors. LV-Fluc-P2A-Neo encodes for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-mNIS-PGK-Puro encodes for mouse Slc5a5 under control of the spleen focus-forming virus (SFFV) promoter and the pac gene under the control of the mouse PGK promoter.
Transfected with: MGI; MGI:2149330; Slc5a5.
Transfected with: UniProtKB; P08659; Firefly luciferase.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac.
Disease Mouse melanoma (NCIt: C21790)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.
Hierarchy Parent: CVCL_0159 (B16-F10)
Sex of cell Male
Category Cancer cell line
Cell line collections Imanis; CL069
Other Wikidata; Q54752468
Entry history
Entry creation05-Mar-2018
Last entry update07-Sep-2018
Version number3