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Cellosaurus B16F10-mNIS-Neo/eGFP-Puro (CVCL_QZ93)

[Text version]

Cell line name B16F10-mNIS-Neo/eGFP-Puro
Accession CVCL_QZ93
Resource Identification Initiative To cite this cell line use: B16F10-mNIS-Neo/eGFP-Puro (RRID:CVCL_QZ93)
Comments Characteristics: Reporter cell line, transfected with both the LV-mNIS-P2A-Neo and LV-eGFP-PGK-Puro vectors. LV-mNIS-P2A-Neo encodes for mouse Slc5a5 linked via a P2A cleavage peptide to the neo gene under control of the spleen focus-forming virus (SFFV) promoter. LV-eGFP-PGK-Puro encodes for eGFP under control of the spleen focus-forming virus (SFFV) promoter and the pac gene under the control of the mouse PGK promoter.
Transfected with: MGI; MGI:2149330; Slc5a5.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P13249; S.alboniger pac.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Disease Mouse melanoma (NCIt: C21790)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.
Hierarchy Parent: CVCL_0159 (B16-F10)
Sex of cell Male
Category Cancer cell line
Cell line collections Imanis; CL067
Other Wikidata; Q54752476
Entry history
Entry creation05-Mar-2018
Last entry update07-Sep-2018
Version number2