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Cellosaurus CT26.WT-mNIS-Neo/Fluc-Puro (CVCL_RA07)

Cell line name CT26.WT-mNIS-Neo/Fluc-Puro
Accession CVCL_RA07
Resource Identification Initiative To cite this cell line use: CT26.WT-mNIS-Neo/Fluc-Puro (RRID:CVCL_RA07)
Comments Characteristics: Reporter cell line, transfected with both the LV-mNIS-P2A-Neo and LV-Fluc-P2A-Puro vectors. LV-mNIS-P2A-Neo encodes for mouse Slc5a5 linked via a P2A cleavage peptide to the neo gene under control of the spleen focus-forming virus (SFFV) promoter. LV-Fluc-P2A-Puro encodes for luciferase linked via a P2A cleavage peptide to the pac gene and under control of the spleen focus-forming virus (SFFV) promoter.
Transfected with: MGI; MGI:2149330; Slc5a5.
Transfected with: UniProtKB; P08659; Firefly luciferase.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Transformant: ChEBI; CHEBI:82373; N-nitroso-N-methylurethane (NNMU).
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Gene deletion; MGI; MGI:104738; Cdkn2a; Zygosity=Homozygous (from parent cell line).
  • Mutation; MGI; MGI:96680; Kras; Simple; p.Gly12Asp (c.35G>A); Zygosity=Homozygous (from parent cell line).
Disease Mouse colon adenocarcinoma (NCIt: C120044)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
Hierarchy Parent: CVCL_7256 (CT26.WT)
Sex of cell Female
Category Cancer cell line
Cross-references
Cell line collections (Providers) Imanis; CL047
Encyclopedic resources Wikidata; Q54814720
Entry history
Entry creation05-Mar-2018
Last entry update29-Jun-2023
Version number8