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Cellosaurus HT1080-hNIS-Neo/eGFP-Puro (CVCL_RA13)

[Text version]
Cell line name HT1080-hNIS-Neo/eGFP-Puro
Accession CVCL_RA13
Resource Identification Initiative To cite this cell line use: HT1080-hNIS-Neo/eGFP-Puro (RRID:CVCL_RA13)
Comments Population: Caucasian.
Characteristics: Reporter cell line, transfected with both the LV-hNIS-IRES-Neo and LV-eGFP-PGK-Puro vectors. LV-hNIS-IRES-Neo encodes for human SLC5A5 linked via an internal ribosomal entry site (IRES) to the Neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-eGFP-PGK-Puro encodes for eGFP under control of the spleen focus-forming virus (SFFV) promoter and the pac gene under the control of the mouse PGK promoter.
Transfected with: HGNC; 11040; SLC5A5.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Bone, pelvis, acetabulum; UBERON=UBERON_0001269.
Sequence variations
  • Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease Fibrosarcoma (NCIt: C3043)
Fibrosarcoma (ORDO: Orphanet_2030)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0317 (HT-1080)
Sex of cell Male
Age at sampling 35Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Imanis; CL103
Cell line databases/resources cancercelllines; CVCL_RA13
Encyclopedic resources Wikidata; Q54896561
Entry history
Entry creation05-Mar-2018
Last entry update05-Oct-2023
Version number13