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Cellosaurus Hepa1-6-Fluc-Neo/eGFP-Puro (CVCL_RA20)

[Text version]

Cell line name Hepa1-6-Fluc-Neo/eGFP-Puro
Accession CVCL_RA20
Resource Identification Initiative To cite this cell line use: Hepa1-6-Fluc-Neo/eGFP-Puro (RRID:CVCL_RA20)
Comments Characteristics: Reporter cell line, transfected with both the LV-Fluc-P2A-Neo and LV-eGFP-PGK-Puro vectors. LV-Fluc-P2A-Neo encodes for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-eGFP-PGK-Puro encodes for eGFP under control of the spleen focus-forming virus (SFFV) promoter and the pac gene under the control of the mouse PGK promoter.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P08659; Firefly luciferase.
Transfected with: UniProtKB; P13249; S.alboniger pac.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Disease Hepatocellular carcinoma of the mouse (NCIt: C38756)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Hierarchy Parent: CVCL_0327 (Hepa 1-6)
Sex of cell Female
Category Cancer cell line
Cell line collections Imanis; CL133
Other Wikidata; Q54882824
Entry history
Entry creation05-Mar-2018
Last entry update07-Sep-2018
Version number2