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Cellosaurus HCT 116 CMV-OsTIR1 (CVCL_RJ11)

[Text version]

Cell line name HCT 116 CMV-OsTIR1
Synonyms HCT116 CMV-OsTIR1
Accession CVCL_RJ11
Resource Identification Initiative To cite this cell line use: HCT 116 CMV-OsTIR1 (RRID:CVCL_RJ11)
Comments Characteristics: Parent cell line to study the rapid depletion of a protein. The expressed rice TIR1 can form a functional SCF(OsTIR1) E3 ligase complex with the endogenous components in human cells. In the presence of auxin, a protein of interest fused with mAID is rapidly degraded upon poly-ubiquitylation.
Characteristics: The CMV-OsTIR1-PRO (pMK232) vector was introduced at the both alleles of the AAVS1 locus by using CRISPR/Cas9.
Transfected with: UniProtKB; Q7XVM8; Oryza sativa subsp. japonica TIR1-like protein Os04g0395600.
Transfected with: UniProtKB; P13249; S.alboniger pac.
Sequence variations Homozygous for ACVR2A p.Lys437fs*5 (c.1310delA) (from parent cell line).
Heterozygous for BRCA2 p.Ile2675fs*6 (c.8021_8022insA) (from parent cell line).
Heterozygous for CDKN2A p.Arg24fs*20 (c.68_69insG) and p.Glu74fs*15 (c.220delG) (from parent cell line).
Heterozygous for CTNNB1 p.Ser45del (c.133_135delTCT) (from parent cell line).
Heterozygous for KRAS p.Gly13Asp (c.38G>A) (from parent cell line).
Heterozygous for PIK3CA p.His1047Arg (c.3140A>G) (from parent cell line).
Homozygous for TGFBR2 p.Lys128Serfs*35 (c.383delA) (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0291 (HCT 116)
Sex of cell Male
Age at sampling 48Y
Category Cancer cell line
Publications

PubMed=27052166; DOI=10.1016/j.celrep.2016.03.001
Natsume T., Kiyomitsu T., Saga Y., Kanemaki M.T.
Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors.
Cell Rep. 15:210-218(2016)

Cross-references
Cell line collections RCB; RCB4662
Other Wikidata; Q54881927
Entry history
Entry creation05-Mar-2018
Last entry updated07-Sep-2018
Version number3