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Cellosaurus U2OS His10-SUMO-2-K0-Q87R (CVCL_RJ63)

[Text version]

Cell line name U2OS His10-SUMO-2-K0-Q87R
Accession CVCL_RJ63
Resource Identification Initiative To cite this cell line use: U2OS His10-SUMO-2-K0-Q87R (RRID:CVCL_RJ63)
Comments Characteristics: Transfected with a decahistidine (His10) tagged lysine-deficient (K0) and Q87R SUMO2 and GFP separated by an internal ribosome entry site (IRES).
Transfected with: HGNC; 11125; SUMO2 (with p.Lys11Arg, p.Lys32Arg, p.Lys32Arg, p.Lys34Arg, p.Lys41Arg, p.Lys44Arg and p.Gln87Arg).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Omics: Sumoylated proteins analysis by proteomics.
Derived from sampling site: Bone; tibia.
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=25755297; DOI=10.1074/mcp.O114.044792
Xiao Z., Chang J.-G., Hendriks I.A., Sigurethsson J.O., Olsen J.V., Vertegaal A.C.O.
System-wide analysis of sumoylation dynamics in response to replication stress reveals novel small ubiquitin-like modified target proteins and acceptor lysines relevant for genome stability.
Mol. Cell. Proteomics 14:1419-1434(2015)

PubMed=28112733; DOI=10.1038/nsmb.3366
Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.O., Nielsen M.L.
Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation.
Nat. Struct. Mol. Biol. 24:325-336(2017)

Cross-references
Other Wikidata; Q54973666
Proteomic databases PRIDE; PXD004927
PRIDE; PXD005296
Entry history
Entry creation05-Mar-2018
Last entry update12-Mar-2020
Version number3