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Cellosaurus K-562 CRISPRa (CVCL_RY26)

[Text version]

Cell line name K-562 CRISPRa
Synonyms K562 dCas9-SunTag-VP64
Accession CVCL_RY26
Resource Identification Initiative To cite this cell line use: K-562 CRISPRa (RRID:CVCL_RY26)
Comments Characteristics: This cell line is transfected by a nuclease-deficient mutant of SpCas9 (dCas9) under the control of a promoter containing 4 copies of the activating domain of HSV-1 VP16 (termed VP64) and to 24 copies of a short peptide epitope (from yeast GCN4) which is termed SunTag.
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Derived from sampling site: Pleural effusion.
Sequence variations BCR-ABL1 gene fusion (from parent cell line).
Homozygous for TP53 p.Gln136fs*13 (c.406_407insC) (from parent cell line).
Disease Chronic myelogenous leukemia, BCR-ABL1 positive (NCIt: C3174)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0004 (K-562)
Children:
CVCL_RY28 (K-562 CRISPRa+SaCas9 clone C11)CVCL_RY27 (K-562 CRISPRa+SaCas9 clone C7)
Sex of cell Female
Age at sampling 53Y
Category Cancer cell line
Publications

PubMed=25307933; DOI=10.1016/j.cell.2014.09.039
Tanenbaum M.E., Gilbert L.A., Qi L.S., Weissman J.S., Vale R.D.
A protein-tagging system for signal amplification in gene expression and fluorescence imaging.
Cell 159:635-646(2014)

Cross-references
Other Wikidata; Q54899214
Entry history
Entry creation14-May-2018
Last entry updated05-Jul-2019
Version number3