ID   K-562 CRISPRa
AC   CVCL_RY26
SY   K562 dCas9-SunTag-VP64
DR   Wikidata; Q54899214
RX   PubMed=25307933;
CC   Population: Caucasian.
CC   Characteristics: This cell line is transfected by a nuclease-deficient mutant of SpCas9 (dCas9) under the control of a promoter containing 4 copies of the activating domain of HSV-1 VP16 (termed VP64) and to 24 copies of a short peptide epitope (from yeast GCN4) which is termed SunTag.
CC   Sequence variation: Gene fusion; HGNC; 76; ABL1 + HGNC; 1014; BCR; Name(s)=BCR-ABL1, BCR-ABL (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Gln136fs*13 (c.406_407insC); Zygosity=Homozygous (from parent cell line).
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala = dCas9).
CC   Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
DI   NCIt; C9110; Blast phase chronic myelogenous leukemia, BCR-ABL1 positive
DI   ORDO; Orphanet_521; Chronic myeloid leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0004 ! K-562
SX   Female
AG   53Y
CA   Cancer cell line
DT   Created: 14-05-18; Last updated: 30-01-24; Version: 11
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RX   PubMed=25307933; DOI=10.1016/j.cell.2014.09.039;
RA   Tanenbaum M.E., Gilbert L.A., Qi L.S., Weissman J.S., Vale R.D.;
RT   "A protein-tagging system for signal amplification in gene expression
RT   and fluorescence imaging.";
RL   Cell 159:635-646(2014).
//