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Cellosaurus NMuMG/Fucci2 (CVCL_U135)

[Text version]

Cell line name NMuMG/Fucci2
Synonyms NMuMG/Fucci2 #5; NMuMG-Fucci2
Accession CVCL_U135
Resource Identification Initiative To cite this cell line use: NMuMG/Fucci2 (RRID:CVCL_U135)
Comments Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci2 (Fluorescent Ubiquitination-based Cell Cycle Indicator 2) construct.
Characteristics: Transfected with the Fucci2 probe which consist of the fusion of mCherry) to the ubiquitination domain of human CDT1 (aa 30-120) and of monomeric Venus (mVenus) to the ubiquitination domain of human geminin (aa 1-110).
Transfected with: HGNC; 24576; CDT1 (with p.Met1_Thr29del and p.Ser121_Leu546del).
Transfected with: HGNC; 17493; GMNN (with p.Tyr111_Ile209del).
Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: Namru.
Hierarchy Parent: CVCL_0075 (NMuMG)
Sex of cell Female
Category Spontaneously immortalized cell line

PubMed=21226962; DOI=10.1186/1471-2121-12-2
Sakaue-Sawano A., Kobayashi T., Ohtawa K., Miyawaki A.
Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication.
BMC Cell Biol. 12:2-2(2011)

PubMed=24786503; DOI=10.1101/pdb.prot080408
Sakaue-Sawano A., Miyawaki A.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progressions with Fucci technology.
Cold Spring Harb. Protoc. 2014:Pdb.prot080408-Pdb.prot080408(2014)

Cell line collections RCB; RCB2868
Ontologies CLO; CLO_0050968
Other Wikidata; Q54930851
Entry history
Entry creation03-Feb-2014
Last entry update24-May-2019
Version number5