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Cellosaurus H1 Oct4-EGFP (CVCL_U159)

[Text version]
Cell line name H1 Oct4-EGFP
Synonyms H1 OCT4-EGFP; WA01 Oct4-eGFP Knock In; H1-OGN
Accession CVCL_U159
Resource Identification Initiative To cite this cell line use: H1 Oct4-EGFP (RRID:CVCL_U159)
Comments Characteristics: A eGFP-neo construct was added to the C-terminal of the POU5F1/OCT4 gene by homologous recombination.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C813 (H1.1)
Children:
CVCL_C962 (dH1cf16-iPS-1)CVCL_C963 (dH1cf16-iPS-5)CVCL_C964 (dH1cf32-iPS-2)
CVCL_C965 (dH1cf32-iPS-4)CVCL_C966 (dH1f-iPS3-12)CVCL_C967 (dH1f-iPS3-3)
CVCL_C697 (dH1f-RiPS-1.11)CVCL_C698 (dH1f-RiPS-1.13)CVCL_C699 (dH1f-RiPS-1.14)
CVCL_C700 (dH1f-RiPS-1.2)CVCL_C701 (dH1f-RiPS-1.3)CVCL_C702 (dH1f-RiPS-1.5)
CVCL_C703 (dH1f-RiPS-1.6)CVCL_C704 (dH1f-RiPS-1.7)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): WiCell

Markers:
AmelogeninX,Y
CSF1PO12,13
D5S8189,11
D7S8208,12
D13S3178,11
D16S5399,13
TH019.3
TPOX8,11
vWA15,17

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Publications

PubMed=12577066; DOI=10.1038/nbt788
Zwaka T.P., Thomson J.A.
Homologous recombination in human embryonic stem cells.
Nat. Biotechnol. 21:319-321(2003)

PubMed=18157115; DOI=10.1038/nature06534
Park I.-H., Zhao R., West J.A., Yabuuchi A., Huo H.-G., Ince T.A., Lerou P.H., Lensch M.W., Daley G.Q.
Reprogramming of human somatic cells to pluripotency with defined factors.
Nature 451:141-146(2008)

Cross-references
Cell line collections (Providers) WiCell; h1-oct4-egfp
Cell line databases/resources ISCR; 2081
Encyclopedic resources Wikidata; Q54871906
Entry history
Entry creation03-Feb-2014
Last entry update29-Jun-2023
Version number12