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Cellosaurus HeLa/Fucci2 (CVCL_U187)

[Text version]

Cell line name HeLa/Fucci2
Synonyms HeLa/Fucci2 #3; HeLa.S-Fucci2; HeLa/Fucci(SA)2
Accession CVCL_U187
Resource Identification Initiative To cite this cell line use: HeLa/Fucci2 (RRID:CVCL_U187)
Comments Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci2 (Fluorescent Ubiquitination-based Cell Cycle Indicator 2) construct.
Characteristics: Transfected with the Fucci2 probe which consist of the fusion of mCherry) to the ubiquitination domain of human CDT1 (aa 30-120) and of monomeric Venus (mVenus) to the ubiquitination domain of human geminin (aa 1-110).
Transfected with: HGNC; 24576; CDT1 (with p.Met1_Thr29del and p.Ser121_Leu546del).
Transfected with: HGNC; 17493; GMNN (with p.Tyr111_Ile209del).
Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line

PubMed=21226962; DOI=10.1186/1471-2121-12-2
Sakaue-Sawano A., Kobayashi T., Ohtawa K., Miyawaki A.
Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication.
BMC Cell Biol. 12:2-2(2011)

PubMed=29107535; DOI=10.1016/j.molcel.2017.10.001
Sakaue-Sawano A., Yo M., Komatsu N., Hiratsuka T., Kogure T., Hoshida T., Goshima N., Matsuda M., Miyoshi H., Miyawaki A.
Genetically encoded tools for optical dissection of the mammalian cell cycle.
Mol. Cell 68:626-640(2017)

Cell line collections RCB; RCB2867
Ontologies CLO; CLO_0050932
Other Wikidata; Q54882692
Entry history
Entry creation03-Feb-2014
Last entry updated24-May-2019
Version number7