ID   HeLa/Fucci2
AC   CVCL_U187
SY   HeLa/Fucci2 #3; HeLa.S-Fucci2; HeLa/Fucci(SA)2
DR   CLO; CLO_0050932
DR   RCB; RCB2867
DR   Wikidata; Q54882692
RX   PubMed=21226962;
RX   PubMed=29107535;
CC   Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci2 (Fluorescent Ubiquitination-based Cell Cycle Indicator 2) construct.
CC   Characteristics: Transfected with the Fucci2 probe which consist of the fusion of mCherry) to the ubiquitination domain of human CDT1 (aa 30-120) and of monomeric Venus (mVenus) to the ubiquitination domain of human geminin (aa 1-110).
CC   Transfected with: HGNC; 24576; CDT1 (with p.Met1_Thr29del and p.Ser121_Leu546del).
CC   Transfected with: HGNC; 17493; GMNN (with p.Tyr111_Ile209del).
CC   Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
DI   NCIt; C27677; Human papillomavirus-related endocervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens
HI   CVCL_0030 ! HeLa
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 03-02-14; Last updated: 24-05-19; Version: 7
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RX   PubMed=21226962; DOI=10.1186/1471-2121-12-2;
RA   Sakaue-Sawano A., Kobayashi T., Ohtawa K., Miyawaki A.;
RT   "Drug-induced cell cycle modulation leading to cell-cycle arrest,
RT   nuclear mis-segregation, or endoreplication.";
RL   BMC Cell Biol. 12:2-2(2011).
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RX   PubMed=29107535; DOI=10.1016/j.molcel.2017.10.001;
RA   Sakaue-Sawano A., Yo M., Komatsu N., Hiratsuka T., Kogure T.,
RA   Hoshida T., Goshima N., Matsuda M., Miyoshi H., Miyawaki A.;
RT   "Genetically encoded tools for optical dissection of the mammalian
RT   cell cycle.";
RL   Mol. Cell 68:626-640(2017).
//