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Cellosaurus MAC-T (CVCL_U226)

[Text version]
Cell line name MAC-T
Synonyms Mac-T; MacT; MAC-T3; Mammary Alveolar Cells-large T antigen
Accession CVCL_U226
Resource Identification Initiative To cite this cell line use: MAC-T (RRID:CVCL_U226)
Comments Group: Patented cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10274.
Doubling time: ~17 hours (PubMed=1659986); 48 hours (Note=In serum-free medium) (PubMed=7540186).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=tsA58].
Omics: Transcriptome analysis by microarray.
Anecdotal: Nexia, a former Canadian biotech company was orignally developed to make lactose-free free milk using MAC-T.
Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
Cell type: Mammary alveolar epithelial cell; CL=CL_0002325.
Species of origin Bos taurus (Bovine) (NCBI Taxonomy: 9913)
Breed/subspecies: Holstein.
Hierarchy Parent: CVCL_B3UP (LMH17)
Children:
CVCL_VN97 (MAC-T BLV)CVCL_U230 (MAC-T CU-1)CVCL_U231 (MAC-T CU-2)
CVCL_U232 (MAC-T CU-3)CVCL_HG41 (MD-IGF-I)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

CLPUB00665
Huynh H.T.
Establishment of bovine mammary epithelial cell lines: an in vitro model for lactation.
Thesis MSc (1990), McGill University Montreal, Canada

PubMed=1659986; DOI=10.1016/0014-4827(91)90422-Q
Huynh H.T., Robitaille G., Turner J.D.
Establishment of bovine mammary epithelial cells (MAC-T): an in vitro model for bovine lactation.
Exp. Cell Res. 197:191-199(1991)

PubMed=1423654; DOI=10.1016/S0309-1651(06)80052-4
Rejman J.J., Oliver S.P., Muenchen R.A., Turner J.D.
Proliferation of the MAC-T bovine mammary epithelial cell line in the presence of mammary secretion whey proteins.
Cell Biol. Int. Rep. 16:993-1001(1992)

Patent=US5227301
Turner J.D., Huynh H.T.
Immortalized bovine mammary epithelial cell line.
Patent number US5227301, 13-Jul-1993

Patent=US5455164
Turner J.D.
Ruminant immortalized mammary epithelial cell lines.
Patent number US5455164, 03-Oct-1995

PubMed=7540186; DOI=10.3168/jds.S0022-0302(95)76662-0
Zavizion B., Gorewit R.C., Politis I.
Subcloning the MAC-T bovine mammary epithelial cell line: morphology, growth properties, and cytogenetic analysis of clonal cells.
J. Dairy Sci. 78:515-527(1995)

PubMed=14550508; DOI=10.1016/S0739-7240(03)00062-6
Berry S.D.K., Weber Nielsen M.S., Sejrsen K., Pearson R.E., Boyle P.L., Akers R.M.
Use of an immortalized bovine mammary epithelial cell line (MAC-T) to measure the mitogenic activity of extracts from heifer mammary tissue: effects of nutrition and ovariectomy.
Domest. Anim. Endocrinol. 25:245-253(2003)

DOI=10.4172/2329-888X.1000103
Hosseini A., Sharma R., Bionaz M., Loor J.J.
Transcriptomics comparisons of Mac-T cells versus mammary tissue during late pregnancy and peak lactation.
Adv. Dairy Res. 1:103-103(2013)

PubMed=26659725; DOI=10.1152/ajpcell.00261.2015
Arevalo Turrubiarte M., Perruchot M.-H., Finot L., Mayeur F., Dessauge F.
Phenotypic and functional characterization of two bovine mammary epithelial cell lines in 2D and 3D models.
Am. J. Physiol. Cell Physiol. 310:C348-C356(2016)

PubMed=30055215; DOI=10.1016/j.virusres.2018.07.013
Martinez Cuesta L., Nieto Farias M.V., Lendez P.A., Barone L., Perez S.E., Dolcini G.L., Ceriani M.C.
Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV).
Virus Res. 256:11-16(2018)

Cross-references
Cell line collections (Providers) BCRJ; 0315
Encyclopedic resources Wikidata; Q54903957
Gene expression databases GEO; GSE46476
Entry history
Entry creation03-Feb-2014
Last entry update05-Oct-2023
Version number16