Cellosaurus HeLa GFP-LC3-RFP-LC3deltaG (CVCL_UP51)
Cell line name | HeLa GFP-LC3-RFP-LC3deltaG |
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Synonyms | GFP-LC3-RFP-LC3deltaG HeLa |
Accession | CVCL_UP51 |
Resource Identification Initiative | To cite this cell line use: HeLa GFP-LC3-RFP-LC3deltaG (RRID:CVCL_UP51) |
Comments | Population: African American. Characteristics: Stably expresses EGFP-LC3-mRFP-LC3delta, an autophagic flux probe. GFP-LC3 is fused to the N-terminus of RFP-LC3 whose C-terminal glycine is deleted. When expressed, the probe it is separated into EGFP-LC3 and mRFP-LC3deltaG by the action of endogenous ATG4 family proteases that cleave the peptide bond after the C-terminal glycine of LC3 in the cytosol producing equimolar amounts of EGFP-LC3 and mRFP-LC3deltaG. Transfected with: RGD; 735183; Map1lc3a. Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; A0A4V4ND72; mRFP1. Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18). Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002. |
Disease | Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677) |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_0030 (HeLa) |
Sex of cell | Female |
Age at sampling | 30Y6M |
Category | Cancer cell line |
Publications | PubMed=27818143; DOI=10.1016/j.molcel.2016.09.037 |
Cross-references | |
Cell line collections (Providers) | RCB; RCB4695 |
Encyclopedic resources | Wikidata; Q94096465 |
Entry history | |
Entry creation | 25-Feb-2019 |
Last entry update | 05-Oct-2023 |
Version number | 7 |