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Cellosaurus HCLE (CVCL_UZ28)

[Text version]
Cell line name HCLE
Synonyms HuCl-22/cdk4R/p53DD/TERT
Accession CVCL_UZ28
Resource Identification Initiative To cite this cell line use: HCLE (RRID:CVCL_UZ28)
Comments Transfected with: HGNC; 1773; CDK4 (with p.Arg24Cys).
Transfected with: HGNC; 11730; TERT.
Transfected with: MGI; MGI:98834; Trp53 (with p.Leu14_Lys302del = p53DD).
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Eye, cornea, limbus; UBERON=UBERON_0006761.
Cell type: Keratinocyte; CL=CL_0000312.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_UZ26 (HuCL-22)
Sex of cell Male
Age at sampling 30Y
Category Telomerase immortalized cell line
STR profile Source(s): PubMed=29787296

Markers:
AmelogeninX,Y
CSF1PO12,13
D3S135815,16
D5S81811,12
D7S82010,11
D8S117911,13
D13S31710,12
D16S53911,13
D18S5114,16
D21S1131
FGA19,24
Penta D11,16
Penta E7
TH016,9
TPOX8
vWA16,17

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Web pages https://dermatology.bwh.harvard.edu/docs/Cell-Culture-Core-Cell-Lines.pdf
Publications

PubMed=12077343; DOI=10.1128/mcb.22.14.5157-5172.2002
Rheinwald J.G., Hahn W.C., Ramsey M.R., Wu J.Y., Guo Z.-Y., Tsao H., De Luca M., Catricala C., O'Toole K.M.
A two-stage, p16(INK4A)- and p53-dependent keratinocyte senescence mechanism that limits replicative potential independent of telomere status.
Mol. Cell. Biol. 22:5157-5172(2002)

PubMed=12766048; DOI=10.1167/iovs.02-0851
Gipson I.K., Spurr-Michaud S., Argueso P., Tisdale A., Ng T.F., Russo C.L.
Mucin gene expression in immortalized human corneal-limbal and conjunctival epithelial cell lines.
Invest. Ophthalmol. Vis. Sci. 44:2496-2506(2003)

PubMed=29787296; DOI=10.1080/02713683.2018.1480043
McDermott A.M., Baidouri H., Woodward A.M., Kam W.R., Liu Y., Chen X.-M., Ziemanski J.F., Vistisen K., Hazlett L.D., Nichols K.K., Argueso P., Sullivan D.A.
Short tandem repeat (STR) profiles of commonly used human ocular surface cell lines.
Curr. Eye Res. 43:1097-1101(2018)

Cross-references
Encyclopedic resources Wikidata; Q93984540
Entry history
Entry creation25-Feb-2019
Last entry update05-Oct-2023
Version number9