Cellosaurus CCRF-CEM R30/6 (CVCL_V323)

Cell line name CCRF-CEM R30/6
Accession CVCL_V323
Resource Identification Initiative To cite this cell line use: CCRF-CEM R30/6 (RRID:CVCL_V323)
Comments Selected for resistance to: ChEBI; CHEBI:44185; Methotrexate (MTX).
Sequence variation: Heterozygous for FLT3 p.Ala627Thr (from parent cell line).
Sequence variation: Heterozygous for KRAS p.Gly12Asp (from parent cell line).
Sequence variation: Heterozygous for TP53 p.Arg175His and p.Arg248Gln (from parent cell line).
Derived from sampling site: Peripheral blood.
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0207 (CCRF-CEM)
CVCL_V325 (CCRF-CEM R30dm)
Sex of cell Female
Age at sampling 3Y11M
Category Cancer cell line

Pizzorno G., Mini E., Coronnello M., McGuire J.J., Moroson B.A., Cashmore A.R., Dreyer R.N., Lin J.T., Mazzei T., Periti P., Bertino J.R.
Impaired polyglutamylation of methotrexate as a cause of resistance in CCRF-CEM cells after short-term, high-dose treatment with this drug.
Cancer Res. 48:2149-2155(1988)