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Cellosaurus HUDEP-2 (CVCL_VI06)

[Text version]

Cell line name HUDEP-2
Synonyms HUDEP2; Human Umbilical cord blood-Derived Erythroid Progenitor-2
Accession CVCL_VI06
Resource Identification Initiative To cite this cell line use: HUDEP-2 (RRID:CVCL_VI06)
Comments Population: Japanese.
Characteristics: After in vitro induction of differentiation, will produce enucleated red blood cells.
Doubling time: 22.7 +.- 5.0 hours (PubMed=31753923).
Transfected with: UniProtKB; P03126; Human papillomavirus type 16 protein E6.
Transfected with: UniProtKB; P03129; Human papillomavirus type 16 protein E7.
Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16) [E6/E7].
Miscellaneous: Please contact directly Dr. Nakamura Y. for the use of this cell line (yukionak@brc.riken.jp).
Derived from sampling site: Umbilical cord blood. Cell type=Erythroid progenitor cell.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_YT59 (ssHUDEP2 )
Sex of cell Sex unspecified
Age at sampling <1D
Category Transformed cell line
Publications

PubMed=23533656; DOI=10.1371/journal.pone.0059890
Kurita R., Suda N., Sudo K., Miharada K., Hiroyama T., Miyoshi H., Tani K., Nakamura Y.
Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.
PLoS ONE 8:E59890-E59890(2013)

Patent=US8975072
Nakamura Y., Kurita R.
Human erythroid progenitor cell line comprising HPV E6/E7 operably linked to an inducible promoter and method for producing human enucleated red blood cells.
Patent number US8975072, 10-Mar-2015

PubMed=31164570; DOI=10.3390/mps1030028
Moir-Meyer G., Cheong P.L., Olijnik A.-A., Brown J., Knight S., King A., Kurita R., Nakamura Y., Gibbons R.J., Higgs D.R., Buckle V.J., Babbs C.
Robust CRISPR/Cas9 genome editing of the HUDEP-2 erythroid precursor line using plasmids and single-stranded oligonucleotide donors.
Methods Protoc. 1:28.1-28.17(2018)

PubMed=31753923; DOI=10.3324/haematol.2019.229211
Daniels D.E., Downes D.J., Ferrer-Vicens I., Ferguson D.C.J., Singleton B.K., Wilson M.C., Trakarnsanga K., Kurita R., Nakamura Y., Anstee D.J., Frayne J.
Comparing the two leading erythroid lines BEL-A and HUDEP-2.
Haematologica 105:E389-E394(2020)

Cross-references
Cell line collections RCB; RCB4557
Encyclopedic resources Wikidata; Q54896730
Entry history
Entry creation14-May-2018
Last entry update17-Mar-2022
Version number8