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Cellosaurus HeLa/Fucci(CA)2 (CVCL_VM98)

[Text version]

Cell line name HeLa/Fucci(CA)2
Synonyms HeLa/Fucci(CA)2 #3
Accession CVCL_VM98
Resource Identification Initiative To cite this cell line use: HeLa/Fucci(CA)2 (RRID:CVCL_VM98)
Comments Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci(CA2) (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct.
Characteristics: Transfected with the Fucci(CA)2 probe which consist of the fusion of mCherry) to the ubiquitination domain of human CDT1 (aa 1-100) but with the Cy motif 68-RRL-70 replaced by AAA and of monomeric Venus (mVenus) to the ubiquitination domain of human geminin (aa 1-110).
Transfected with: HGNC; 24576; CDT1 (with p.Ser101_Leu546del and p.Arg68Ala, p.Arg69Ala and p.Leu70Ala).
Transfected with: HGNC; 17493; GMNN (with p.Tyr111_Ile209del).
Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line

PubMed=29107535; DOI=10.1016/j.molcel.2017.10.001
Sakaue-Sawano A., Yo M., Komatsu N., Hiratsuka T., Kogure T., Hoshida T., Goshima N., Matsuda M., Miyoshi H., Miyawaki A.
Genetically encoded tools for optical dissection of the mammalian cell cycle.
Mol. Cell 68:626-640(2017)

Cell line collections RCB; RCB4676
Other Wikidata; Q94101207
Entry history
Entry creation07-Sep-2018
Last entry update02-Jul-2020
Version number3