ID   HeLa/Fucci(CA)2
SY   HeLa/Fucci(CA)2 #3
DR   RCB; RCB4676
DR   Wikidata; Q94101207
RX   PubMed=29107535;
CC   Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci(CA2) (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct.
CC   Characteristics: Transfected with the Fucci(CA)2 probe which consist of the fusion of mCherry) to the ubiquitination domain of human CDT1 (aa 1-100) but with the Cy motif 68-RRL-70 replaced by AAA and of monomeric Venus (mVenus) to the ubiquitination domain of human geminin (aa 1-110).
CC   Transfected with: HGNC; 24576; CDT1 (with p.Ser101_Leu546del and p.Arg68Ala, p.Arg69Ala and p.Leu70Ala).
CC   Transfected with: HGNC; 17493; GMNN (with p.Tyr111_Ile209del).
CC   Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
DI   NCIt; C27677; Human papillomavirus-related endocervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens
HI   CVCL_0030 ! HeLa
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 07-09-18; Last updated: 02-07-20; Version: 3
RX   PubMed=29107535; DOI=10.1016/j.molcel.2017.10.001;
RA   Sakaue-Sawano A., Yo M., Komatsu N., Hiratsuka T., Kogure T.,
RA   Hoshida T., Goshima N., Matsuda M., Miyoshi H., Miyawaki A.;
RT   "Genetically encoded tools for optical dissection of the mammalian
RT   cell cycle.";
RL   Mol. Cell 68:626-640(2017).