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Cellosaurus HeLa/Fucci(SCA)2 (CVCL_VM99)

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Cell line name HeLa/Fucci(SCA)2
Accession CVCL_VM99
Resource Identification Initiative To cite this cell line use: HeLa/Fucci(SCA)2 (RRID:CVCL_VM99)
Comments Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci(SCA2) (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct.
Characteristics: Transfected with the Fucci(SCA)2 probe which consist of the fusion of mCherry) to the ubiquitination domain of human CDT1 (aa 1-100) and of monomeric Venus (mVenus) to the ubiquitination domain of human geminin (aa 1-110).
Transfected with: HGNC; 24576; CDT1 (with p.Ser101_Leu546del and p.Arg68Ala, p.Arg69Ala and p.Leu70Ala).
Transfected with: HGNC; 17493; GMNN (with p.Tyr111_Ile209del).
Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Publications

PubMed=29107535; DOI=10.1016/j.molcel.2017.10.001
Sakaue-Sawano A., Yo M., Komatsu N., Hiratsuka T., Kogure T., Hoshida T., Goshima N., Matsuda M., Miyoshi H., Miyawaki A.
Genetically encoded tools for optical dissection of the mammalian cell cycle.
Mol. Cell 68:626-640(2017)

Entry history
Entry creation07-Sep-2018
Last entry updated24-May-2019
Version number2